| Arabidopsis Seed Germination Assay with Gibberellic Acid
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Author:
Date:
2016-11-20
[Abstract] This assay analyzes Arabidopsis seed germination in response to gibberellic acid (GA). During seed imbibition, visible physiological changes allow precise determination of germination rate. This protocol utilizes a stereoscopic microscope to improve characterization of seed germination process.
[摘要] 该测定分析响应于赤霉酸（GA）的拟南芥种子萌发。在种子吸水期间，可见的生理变化允许精确测定发芽率。该协议利用立体显微镜来改善种子发芽过程的表征。
[背景] 种子萌发是由植物激素（如GA和脱落酸（ABA））和环境因素控制的植物生命周期的关键过程。种子萌发包括两个生理过程，包括种皮（睾丸）和胚乳破裂。通常，胚乳通过胚根的渗透表明萌发是完全的。以前的研究通常使用胚乳破裂来计算发芽率。然而，种皮破裂也测量种子发芽的进展。该协议利用立体显微镜提供种子发芽的可见和精确计算，包括种皮和胚乳破裂的速率（Zhong等人，2015）。
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| Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.)
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Author:
Date:
2016-09-05
[Abstract] Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental importance and has become the focus of intensive research. This protocol describes a simple and reproducible protocol to imitate natural salinity and drought stress under soil conditions. Even though the water-culture method is most frequently used for ...
[摘要] 盐度和干旱是导致红麻产量和质量损失的两个主要因素。 据报道，盐度和干旱可影响10％以上的耕地，导致主要作物平均单产下降50％以上。 因此，了解植物对干旱和盐分的耐受性具有根本重要性，已成为深入研究的重点。 该协议描述了在土壤条件下模拟天然盐度和干旱胁迫的简单和可重复的方案。 即使水培法最常用于盐分和干旱处理，本研究中的土壤培养方法更适用于研究自然胁迫条件。
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