| Isolation of Intestinal Mesenchymal Cells from Adult Mice
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Author:
Date:
2016-09-20
[Abstract] During the last 20 years intestinal mesenchymal cells (IMCs) have emerged as an important cell type that plays a central role in intestinal development and homeostasis, by providing both structural support and growth regulatory elements. IMCs also actively participate in wound healing responses, thus regulating pathologic conditions such as tissue repair, inflammation, fibrosis and carcinogenesis (Powell et al., 2011). We have recently demonstrated that intestinal mesenchymal-specific signals play important in vivo physiological roles in intestinal inflammation and ...
[摘要] 在过去20年间，肠间质细胞（IMC）已经作为重要的细胞类型出现，通过提供结构支持和生长调节元件在肠发育和体内平衡中起着中心作用。 IMC还积极参与伤口愈合反应，从而调节病理状况，例如组织修复，炎症，纤维化和癌发生（Powell等人，2011）。 我们最近已经证明肠间充质特异性信号在肠炎症和癌发生中在体内起重要的生理作用（Koliaraki等人，2012; Roulis等人，/em>。，2014; Koliaraki 。。，2015）。 在这里我们描述了用于从成年小鼠肠道分离和培养间充质细胞的酶法。
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| Olfactory Bulb (OB) Transplants
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Author:
Date:
2016-09-05
[Abstract] Transplantation in mouse brain slices is a powerful tool in order to study axon targeting and migrational events during development. Taking advantage of donors and recipients belonging to different genotypes, this technique allows researchers to assess the contribution of donor and/or recipient tissue by performing various combinations and to study cell-autonomous functions or effects that are influenced by the recipient’s environment (Bastakis, et al., 2015). Here we describe the transplantation procedure on sagittal brain slices containing olfactory bulb (OB). Specifically, we have ...
[摘要] 小鼠脑切片中的移植是一个强大的工具，以便研究轴突靶向和发展过程中的迁移事件。 利用属于不同基因型的供体和受体，该技术允许研究人员通过进行各种组合来评估供体和/或受体组织的贡献，并研究受受体环境影响的细胞自主功能或效应（Bastakis， em>等人，2015）。 在这里我们描述的矢状大脑切片包含嗅球（OB）的移植程序。 具体来说，我们已经将背侧OB的近端到外皮部分移植到受体切片上的相同区域。 移植的切片可以培养长达3天，之后由于3D中的生长它们的形态被破坏。 这些切片的重新切片允许更详细的免疫组织化学分析。
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