X-TRACTA Tips

公司名称: Biozym Scientific

产品编号: 615935

Purification of Native Dentilisin Complex from Treponema denticola by Preparative Continuous Polyacrylamide Gel Electrophoresis and Functional Analysis by Gelatin Zymography

Pachiyappan Kamarajan, John C. Timm, M. Paula Goetting-Minesky, Erin T. Malone, Sean Ganther, Allan Radaic, Christian Tafolla, J. Christopher Fenno and Yvonne L. Kapila

Published online: 2024-04-05

Bio-protocol()

Company-protocol()

Other protocol()

Drosophila Fecal Sampling

Author:

Christine Fink, Jakob von Frieling, Mirjam Knop and Thomas Roeder,

Date:

2017-09-20

[Abstract] Fecal sampling is a non-invasive method which raises the possibility to study the development and the changes in the microbial community throughout different time points of a fly population or throughout different treatments. This method allows precise manipulation to trigger the fly’s physiology by nutritional interventions, bacterial infections or other stressors.

As in most other animals, the intestinal microbiota is essential for a healthy fly-life. Because Drosophila only harbors a relative simple bacterial community with a small variety of round about 8 to 10 ... [摘要] 粪便取样是一种非侵入性方法，提高了在飞行群体的不同时间点或整个不同处理过程中研究微生物群落的发展和变化的可能性。这种方法允许精确的操纵通过营养干预，细菌感染或其他压力源触发苍蝇的生理学。
  与大多数其他动物一样，肠道微生物群对健康的飞行生命至关重要。因为果蝇只拥有一个相对简单的细菌群落，有大约8到10个不同种类的小种，很容易建立微生物群落，并在处理后调查微生物的变化。
  使用蝇的粪便的另一个积极作用是不是肠微生物群的一部分的细菌，例如Wolbachia，可以直接从分析中排除，因为它们不会排泄。
  使用这种方法，生成的数据集可能反映了在简单飞行模型中研究微生物组织相关疾病的一个很好的范例，此外，可以以高通量方法测试药物。

产品评论

X-TRACTA Tips

免责声明