Goat F(ab’)2 Fragment anti-mouse IgG (H+L)-FITC

山羊F（ab''）2片段抗小鼠IgG（H + L）-FITC

公司名称: Beckman Coulter

产品编号: PN IM0819

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Peptide Loading on MHC Class I Molecules of Tumor Cells

Author:

Loredana Cifaldi, Franco Locatelli and Doriana Fruci,

Date:

2016-09-20

[Abstract] MHC class I molecules present peptides to cytotoxic T cells allowing the immune system to scan for intracellular pathogens and mutated proteins. The generation of antigenic peptides is a multistep process that ends in the endoplasmic reticulum (ER). Only peptides with the right length and sequence will bind nascent MHC class I molecules in the ER. This protocol allows for detachment of the endogenous peptides bound to MHC class I molecules by preserving them for the binding of high affinity synthetic peptides. The complete dissociation of endogenous peptides by mild acid treatment as well as ... [摘要] MHC I类分子将肽呈递到细胞毒性T细胞，允许免疫系统扫描胞内病原体和突变蛋白。抗原肽的产生是以内质网（ER）结束的多步过程。只有具有正确长度和序列的肽将结合ER中的新生MHC I类分子。该方案允许通过保持结合MHC I类分子的内源肽与高亲和力合成肽的结合而脱离。通过温和酸处理以及合成肽与MHC I类分子的结合来评价内源肽的完全解离，通过流式细胞术测量在细胞表面上表达的HLA I类分子。识别β2m相关HLA-A，-B，-C，-E和-G重链的小鼠抗体W6/32适合于此提议。表达表面HLA I类分子的任何肿瘤细胞系适于测定。另一个重要方面是知道肿瘤细胞系的HLA I类分型以允许选择已知的高亲和力肽。

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Goat F(ab’)2 Fragment anti-mouse IgG (H+L)-FITC

山羊F（ab''）2片段抗小鼠IgG（H + L）-FITC

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