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Author:
Date:
2017-10-20
[Abstract] Reversal learning can be used to examine deficits in cognitive flexibility, which have been linked to a number of neuropsychiatric disorders including schizophrenia and addiction. However, methods of examining reversal learning have varied substantially between species. Touchscreen technology has allowed researchers to explore cognitive deficits with a platform that is translatable across rodents, non-human primates and human subjects. Here we describe a method for measuring visual discrimination and reversal learning in mice using automated touchscreen-based operant chambers.
[摘要]
材料和试剂
Microtube（INA•OPTIKA，Bio-Bik，目录号：ST-0150F）多孔培养板6孔（Greiner Bio One International，目录号：657160）15ml锥形离心管（Greiner Bio One International，目录号：188271）具有0.22μm孔径膜的注射器过滤器（Pall，目录号：4192）HEK293T细胞Notch1表达载体（pTracer-CMV / Notch1 ）（Sawaguchi等人，2017）注意：不可商业使用。 表达载体（pSecTag2 / Hygro / Eogt）的Eogt表达载体（Sawaguchi等人，2017）注意：不可商业使用。GFP表达载体（pMAX-GFP）（Addgene，目录号：VDF-1012）Dynabeads蛋白A（Thermo Fisher Scientific，Invitrogen TM，目录号：10002D）Opti-MEM（Thermo Fisher Scientific，Gibco TM ，目录号：31985070）4％多聚甲醛（和光纯药，目录号：163-20145）胎牛血清（FBS）（Sigma-Aldrich，目录号：172012-500ML）磷酸二氢钠（Na 2 HPO ...
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