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Pacific BlueTM anti-mouse/human CD45R/B220 Antibody

Pacific Blue TM 抗小鼠/人CD45R / B220抗体

公司名称: BioLegend
产品编号: 103230
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Imaging Thick Lymph Node Tissue Sections
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Date:
2016-09-20
[Abstract]  Our protocol describes a simple procedure for imaging thick lymph node sections by 2-photon microscopy. Lymph nodes are sectioned using a vibratome (vibrating microtome) to produce slices of tissue that can then be stained with fluorescently labeled antibodies. The thick tissue sections (150-200 μm depth) allow for the detection of cell clustering that is typically under-represented in thin sections (10-20 μm) used for conventional confocal microscopy. Application of 2-photon microscopy facilitates imaging through the thick volume of the vibratome sections. In combination with automated image ... [摘要]  我们的协议描述了一个简单的程序,通过双光子显微镜成像厚淋巴结切片。 使用vibratome(振动切片机)切割淋巴结以产生组织切片,然后可用荧光标记的抗体染色。 厚组织切片(150-200μm深度)允许检测细胞聚集,其通常在用于常规共聚焦显微镜的薄切片(10-20μm)中表现不足。 双光子显微镜的应用有利于成像通过厚的体积的vibratome部分。 与自动图像处理软件组合,厚的淋巴结横截面图像还有助于在组织的相对大的区域内的细胞事件的定量,从而提供关于感兴趣的细胞事件的空间分布的更清晰的图像(例如 。,T细胞聚类)。 该方法也可以容易地应用于其它组织,例如脾或皮肤。

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