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Recombinant Human Ephrin-A3 Fc Chimera Biotinylated Protein

重组人Ephrin-A3 Fc嵌合体生物素化蛋白

公司名称: R&D Systems
产品编号: BT359
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In vitro Assay for Dendritic Spine Retraction of Hippocampal Neurons with Sparse Labeling
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2016-09-20
[Abstract]  Dendritic spines are the post-synaptic structures that play a central role in excitatory synaptic transmission. Developmental spinogenesis relies on a variety of stimuli such as those derived from cell-cell communication and their downstream signaling. Here, we describe an in vitro assay of dendritic spine retraction using hippocampal slice culture, in which individual neurons are sparsely and brightly labeled by the Supernova method, for the study of molecular mechanisms of spine development. [摘要]  树突状棘是突触后结构,在兴奋性突触传播中起重要作用。 发育性的发生依赖于各种刺激物,如来自细胞 - 细胞通讯及其下游信号传导的刺激。 在这里,我们描述了使用海马片段培养的树突状脊柱退缩的体外测定,其中单个神经元被Supernova方法稀疏和明亮地标记,用于研究脊柱发育的分子机制。

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