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Mirror Units Optics Fluorescence Filter Cubes

荧光镜单元

公司名称: Olympus
产品编号: U-MWIBA3
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Direct Visualization of the Multicopy Chromosomes in Cyanobacterium Synechococcus elongatus PCC 7942
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Date:
2018-08-05
[Abstract]  Cyanobacteria are prokaryotic organisms that carry out oxygenic photosynthesis. The fresh water cyanobacterium Synechococcus elongatus PCC 7942 is a model organism for the study of photosynthesis and gene regulation, and for biotechnological applications. Besides several freshwater cyanobacteria, S. elongatus 7942 also contains multiple chromosomal copies per cell at all stages of its cell cycle. Here, we describe a method for the direct visualization of multicopy chromosomes in S. elongatus 7942 by fluorescence in situ hybridization (FISH). [摘要]  蓝细菌是进行含氧光合作用的原核生物。 淡水蓝藻 Synechococcus elongatus PCC 7942是用于研究光合作用和基因调控以及生物技术应用的模式生物。 除了几种淡水蓝藻, S. 细胞 7942在其细胞周期的所有阶段每个细胞还含有多个染色体拷贝。 在这里,我们描述了一种直接可视化 S中多拷贝染色体的方法。 通过荧光原位杂交(FISH)测定细菌 7942。

【背景】蓝藻是原核微生物,其利用与高等植物中的叶绿体类似的产氧光合系统。虽然 Escherichia coli 和枯草芽孢杆菌等细菌具有单个圆形染色体,但是几种淡水蓝细菌每个细胞有多个圆形染色体(Mann和Carr,1974; Labarre 等人,1989; Binder和Chisholm,1995)。淡水蓝藻 Synechococcus elongatus PCC 7942(以下简称 S.longatus 7942)每个细胞携带3-8个染色体拷贝(Griese et al。,2011; Watanabe et al。,2012; Watanabe et al。,2015)。已经建立了荧光报告子 - 操纵子系统来获取 S的多拷贝染色体的图像。 elongatus 7942(Chen et al。,2012; Jain et ...

Retrograde and Anterograde Tracing of Neural Projections
Author:
Date:
2013-08-20
[Abstract]  Neurons consist of four elements, the soma, dendrite, axon and terminal. They work in concert as the input (soma and dendrite) and output (axon and terminal) parts of neuronal transmission. To function and maintain neuronal activity and metabolisms, proteins and organelles should be transported from soma to terminal via anterograde axonal transport, and also from terminal to soma via retrograde transport. By utilizing these transport systems, neural projection is traced by injecting tracers into local sites of interest. Furthermore, neurochemical properties, such as glutamatergic and ... [摘要]  神经元由四个元素组成,即索马,树突,轴突和末端。 它们作为神经元传播的输入(神经和树突)和输出(轴突和末端)部分协同工作。 为了起到和维持神经元活动和代谢的作用,蛋白质和细胞器应通过逆行轴突运输从脊髓运输到终末,也可以通过逆行运输从末端转移到血管。 通过利用这些运输系统,通过将示踪剂注入到感兴趣的地方来跟踪神经投影。 此外,神经化学性质,如谷氨酸能和GABA能,可以通过逆行和顺行跟踪与荧光原位杂交和免疫荧光结合来确定。

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