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FreeZone 4.5 Liter Benchtop Freeze Dry System

FreeZone 4.5升台式冷冻干燥系统

公司名称: Labconco
产品编号: 7750020
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Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass
Author:
Date:
2017-03-05
[Abstract]  Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic and biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll and Somerville, 2009; Zhao and Dixon, 2011; Wang et al., 2013). For these reasons, studying the properties of lignin is of great interest to researchers in agriculture and bioenergy fields. This protocol describes the acetyl bromide method of total lignin extraction and quantification, which is favored among other ... [摘要]  木质素是地球上第二大丰富的生物聚合物,为植物在二次细胞壁提供机械支持,防止非生物和生物胁迫。然而,木质素还可以作为生物燃料生成的糖化的障碍(Carroll和Somerville,2009; Zhao和Dixon,2011; Wang等人,2013)。由于这些原因,研究木质素的性质对于农业和生物能源领域的研究人员非常感兴趣。该方案描述了总木素提取和定量的乙酰溴方法,其对于其不同组织类型的高回收率,一致性和不敏感性的其它方法是有利的(Johnson等人,1961; Chang < em等人,2008;="" moreira-vilar等人,2014;="">

背景 用于从植物生物量定量木质素的乙酰溴方法已被用于精确测量几十年的总木质素含量(Johnson等,1961)。最近,与替代的巯基乙酸和Klason木质素方法(Moreira-Vilar等人,2014))相比,该方法获得了木质素定量的最佳方法的支持。这三种方法的比较经验证明,乙酰溴方法一直导致木质素回收率最高,对组织类型,木质化程度和木质素组成不敏感(Moreira-Vilar等人)。 ...

Glucosinolates Determination in Tissues of Horseradish Plant
Author:
Date:
2015-08-20
[Abstract]  Glucosinolates (GLS) are secondary metabolites mainly found in plants belonging to the Brassicaceae family, including also horseradish (Armoracia rusticana G. Gaertn., B. Mey. & Scherb), a popular spice with a characteristic pungent flavor due to the abundance of GLS. Such compounds exhibit antibacterial, antifungal, and insecticidal activities, as well as human health properties. Therefore, it is very important to have a full understanding of their levels and profiles in plants. However, the characterization of GLS from horseradish crude extracts is a tough task, due to the complexity of the ... [摘要]  硫代葡萄糖苷(GLS)是次要代谢物,主要在属于十字花科的植物中发现,包括辣根(Armoracia rusticana G.Gaertn,B.Mey。&Scherb),由于GLS丰度而具有特征刺激性风味 。 这种化合物表现出抗菌,抗真菌和杀虫活性以及人类健康性质。 因此,对其在植物中的水平和谱的完全理解是非常重要的。 然而,由于植物基质的复杂性和痕量的许多GLS的出现,来自辣根粗提取物的GLS的表征是艰难的任务。 在这里我们描述两种替代的有效和快速的方法在辣根植物的GLS表征:液相色谱耦合高分辨率质谱(LC-MS)测定完整的GLS和HPLC-UV测定脱硫-GLS。

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