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公司名称: BIO CRAFT
产品编号: BC-700
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Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2
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Date:
2018-10-20
[Abstract]  Tissue clearing techniques are useful for large-scale three-dimensional fluorescence imaging of thick tissues. However, high-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we present a water-based optical clearing and mounting media, SeeDB2, which is designed for high numerical aperture (NA) objective lenses with oil or glycerol immersion. Using quick and simple soaking procedures, the refractive indices of samples can be matched either to that of immersion oil (1.52) or glycerol (1.46), thus ... [摘要]  组织清除技术可用于厚组织的大规模三维荧光成像。然而,高分辨率成像深层组织一直是一个挑战,因为它对光散射和球面像差极为敏感。在这里,我们提出了一种水基光学清除和安装介质SeeDB2,它是专为高数值孔径(NA)物镜和油或甘油浸泡而设计的。使用快速简单的浸泡程序,样品的折射率可以与浸油(1.52)或甘油(1.46)相匹配,从而最大限度地减少光散射和球面像差。在清理和成像过程中,高度保留了良好的形态和各种荧光蛋白。我们的方法可用于使用共聚焦和超分辨率显微镜在突触分辨率下的神经元电路的三维荧光成像。 SeeDB2也可用作荧光蛋白超分辨率成像的封固介质。
【背景】生物组织以3D组织。此外,许多重要的细胞机器,例如,例如,神经元中的突触,是亚微米级的。因此,对用于亚微米级3D成像的方法的需求不断增加。串联电子显微镜技术(例如>,FIB-SEM或SBF-SEM)很有前景,但它们无法充分利用现代生命科学中可用的基因荧光标记工具。为了利用荧光显微镜促进3D成像,近年来已经开发了许多组织清除技术(Richardson和Lichtman,2015和2017)。它们专为大规模3D成像而设计,其中一些可用于全脑,甚至是固定样品的全身尺度荧光成像,结合共焦,双光子或光片显微镜。然而,其中许多尚未针对高分辨率成像进行全面优化。
在荧光显微镜中,横向分辨率( d >)给出如下:
d ...

DNA Fragmentation Analysis
Author:
Date:
2014-08-05
[Abstract]  DNA fragmentation with length corresponding to multiple integer of approximately 180 base pairs is a distinct feature of apoptosis in animals and programmed cell death in plants. This feature can simply be detected by DNA gel electrophoresis followed by ethidium bromide staining, although in some cases it is difficult to distinguish the DNA laddering. We herein describe a protocol to detect a programmed cell death-associated DNA laddering of plant tissues. After agarose-gel electrophoresis of genomic DNA, Southern hybridization using DIG-labeled genomic DNA probe is performed, that improves ... [摘要]  具有对应于大约180个碱基对的多个整数的长度的DNA片段是动物中凋亡和植物中程序性细胞死亡的独特特征。 这个特征可以简单地通过DNA凝胶电泳随后溴化乙锭染色检测,尽管在一些情况下难以区分DNA梯状。 我们在本文中描述了用于检测植物组织的程序性细胞死亡相关DNA梯状的方案。 在基因组DNA的琼脂糖凝胶电泳后,进行使用DIG标记的基因组DNA探针的Southern杂交,其改善DNA梯状的检测。

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