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Blue fluorescence illumination

蓝色荧光照明

公司名称: Olympus
产品编号: U-MGGFPHQ
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Transpharyngeal Exposure of GnRH Neurons
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2014-03-20
[Abstract]  The neurons secreting gonadotropin-releasing hormone (GnRH) control fertility in all mammalian species. However, investigations of this neuronal population are difficult as the cell bodies of the approximately 800 GnRH neurons are scattered through the basal forebrain ranging from the olfactory bulbs through to the base of the hypothalamus. While acute brain slice preparations enable the electrophysiological characteristics of these cells to be determined in vitro, their topography has made investigations in vivo extremely difficult. We detail here a surgical approach that ... [摘要]  分泌促性腺激素释放激素(GnRH)的神经元控制所有哺乳动物物种的生育力。 然而,对于这种神经元群体的研究是困难的,因为大约800个GnRH神经元的细胞体通过从嗅球到下丘脑的基底的基底前脑分散。 虽然急性脑切片制剂使得这些细胞的电生理特征在体外得以确定,但它们的形态使得体内的研究非常困难。 我们在这里详细介绍一种手术方法,允许在麻醉小鼠体内评估下丘脑基部的GnRH神经元(图1)。 该过程使得能够从位于小鼠脑的腹侧表面上的神经元进行电记录(Constantin等人,2013)。

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