Quantitative Determination of Poly-β-hydroxybutyrate in Synechocystis sp. PCC 6803
|
Author:
Date:
2017-07-20
[Abstract] Cyanobacteria synthesize a variety of chemically-different, high-value biopolymers such as glycogen (polyglucose), poly-β-hydroxybutyrate (PHB), cyanophycin (polyamide of arginine and aspartic acid) and volutin (polyphosphate) under excess conditions. Especially under unbalanced C to N ratios, glycogen and in some cyanobacterial genera also PHB are massively accumulated in the progression of the general nitrogen stress response. Several different technologies have been established for in situ and in vitro PHB analysis from different microbial sources. In this ...
[摘要] 蓝细菌合成各种化学上不同的高价值生物聚合物,如糖原(polyglucose),poly-β-β-羟基丁酸酯(PHB),蓝藻素(精氨酸和天冬氨酸的聚酰胺)和挥发物(多磷酸盐) 在超额条件下。 特别是在不平衡的C至N比下,糖原和一些蓝藻属中,PHB在一般氮应激反应进程中大量积累。 已经针对不同微生物来源的原位实验和/或从体外分析,建立了几种不同的技术。 在该协议中,描述了用于蓝藻(Stechocystis)的PHB定量的快速可靠的分光光度法。 如(Damrow等人,2016)中描述的氮缺乏的PCC 6803。 【背景】非重氮营养蓝细菌,例如集胞藻(Synechocystis) PCC 6803通过漂白来解决缺乏组合的氮源,这是一种被称为褪绿的过程(Allen和Smith,1969)。这种驯化反应的特征在于四个主要的结构和形态变化:(i)类囊体层之间的电子致密糖原夹杂物(直径约40nm)的大量积累伴随着(ii)藻糖酵母天线复合物的降解, (iii)类囊体膜层的拆卸,包括数量减少和包装密度,和(iv)形成不同的电子透明PHB颗粒(直径约400-500nm)(Damrow等人,2016)。由于不存在分解代谢酶和PHB缺陷型突变体的明显表型,所以在几种物种中合成的蓝细菌PHB代谢的生理功能是相当不透明的(Beck等人,2012; van ,2010; Damrow等人,2016; ...
|
|
Quantification of Chitinase Activity in Fusarium oxysporum
|
Author:
Date:
2016-08-05
[Abstract] Fungal morphogenetic development requires modification and plasticity of the cell wall, which implies synthesis and remodelling of its components, including chitin and glucan. Thus chitinase and glucanase activities are crucial for cell-wall biogenesis and cell division. Quantification of chitinase activity might be useful to identify structural defects that could negatively influence growth and morphogenesis of some filamentous fungi like Fusarium oxysporum, which produces both intracellular and secreted chitinases. The chitinolytic enzymes are categorized based on their enzymatic ...
[摘要] 真菌形态发生需要细胞壁的修饰和可塑性,这意味着其组分(包括壳多糖和葡聚糖)的合成和重塑。因此几丁质酶和葡聚糖酶活性对于细胞壁生物发生和细胞分裂是至关重要的。几丁质酶活性的定量可能有助于鉴定可能负面影响一些丝状真菌如产生细胞内和分泌型几丁质酶的尖孢镰刀菌的生长和形态发生的结构缺陷。基于它们对壳多糖底物的酶作用将壳聚糖分解酶分类。内酯酶被定义为催化几丁质链中内部点的随机裂解的酶。外切球蛋白酶催化乙酰壳寡糖或N-乙酰葡糖胺从几丁质的非还原端逐步释放,因此分别称为壳聚糖酶和β-N-乙酰氨基葡糖苷酶。在这里,我们描述了一种简单的方法来轻易地纯化几丁质酶,以便比较不同的F的内切几丁质酶活性和外切几丁质酶活性。尖孢镰孢菌株。该方案可适用于任何真菌物种。
|
|