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Author:
Date:
2016-03-20
[Abstract] There is now general agreement that nitric oxide (NO) is an important and almost ubiquitous signal in plants. Nevertheless, there are still many controversial observations and differing opinions on the importance and functions of NO in plants. Partly, this may be due to the difficulties in detecting and quantifying NO. Here, we summarize protocols for detecting NO and quantifying nitrite concentration in Arabidopsis seedlings. We also present a method to measure NO in biofilms formed by the plant growth promoting rhizobacteria Azospirillum brasilense (A. brasilense). ...
[摘要] 现在普遍认为一氧化氮（NO）是植物中重要的和几乎普遍存在的信号。然而，对于植物中NO的重要性和功能仍有许多有争议的观察和不同的观点。部分地，这可能是由于检测中的困难以及甚至更多的在定量NO。在这里，我们总结了在拟南芥幼苗中检测NO和定量亚硝酸盐浓度的方案以及在由植物生长促进根瘤菌（Azospirillum brasilense）形成的生物膜中的NO实时测量（ > A。brasilense ）。含氧水溶液中的NO具有短的半衰期，其通常归因于快速氧化成亚硝酸盐。在这里我们详细的使用荧光探针DAF-FM DA和电化学方法分别直接检测和定量NO，Griess试剂通过其氧化的亚硝酸盐形式间接检测NO。这些方案可用于多种细胞类型和植物的不同组织以及微生物。
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