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Lysozyme

溶菌酶
公司名称: Roche Diagnostics
产品编号: 10153516103
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In vitro DNA Polymerization Activity Assay Using Cell-free Extracts
Author:
Date:
2014-08-20
[Abstract]  This protocol has been designed to measure the in-vitro DNA polymerization activity in crude cell extracts of the Antarctic bacterium Pseudomonas syrinagae Lz4W. This bacterium can grow at 4 °C with optimum growth rate at 22 °C. The slow growth rate of the bacterium observed at low temperature (4 °C) compared to higher temperature (22 °C) can be attributed to the reduced rate of DNA replication at low temperature. Here we describe a protocol which we have used to quantify the in vitro DNA polymerization of cell extracts at two different temperatures. [摘要]  该方案已经设计用于测量南极细菌假单胞菌syrinagae Lz4W的粗细胞提取物中的体外 DNA聚合活性。 该细菌可以在4℃下生长，在22℃以最佳生长速率生长。 在低温（4℃）下观察到的细菌与较高温度（22℃）相比的缓慢生长速率可以归因于在低温下DNA复制的速率降低。 在这里我们描述了一个协议，我们已经用来量化的体外细胞提取物在两个不同的温度下的DNA聚合。

Preparation of Bacillus subtilis Cell Lysates and Membranes
Author:
Date:
2014-01-20
[Abstract]  A common feature of every eukaryotic and prokaryotic cell is that they exhibit a plasma membrane. In Bacillus subtilis (B. subtilis) roughly 25% of all proteins are putative trans- or membrane associated proteins. Here we describe a relatively simple method to separate and prepare membrane and cytosolic proteins by ultra-centrifugation.
 [摘要]  每个真核和原核细胞的共同特征是它们表现出质膜。 在枯草芽孢杆菌（<枯草芽孢杆菌）中，大约25％的蛋白质是推定的跨膜或膜相关蛋白。 在这里，我们描述了一种相对简单的方法，通过超离心分离和制备膜和胞质蛋白

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