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Probenecid

丙磺舒

公司名称: Sigma-Aldrich
产品编号: P9861
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HIV-1 Fusion Assay
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2014-08-20
[Abstract]  The HIV-1 fusion assay measures all steps in the HIV-1 life cycle up to and including viral fusion. It relies on the incorporation of a β-lactamase–Vpr (BlaM-Vpr) protein chimera into the virion and the subsequent transfer of this chimera into the target cell by fusion (Figure 1). The transfer is monitored by the enzymatic cleavage of CCF2, a fluorescent dye substrate of β-lactamase, loaded into the target cells. Cleavage of the β-lactam ring in CCF2 by β-lactamase changes the fluorescence emission spectrum of the dye from green (520 nm) to blue (447 nm). This change reflects virion fusion ... [摘要]  HIV-1融合测定测量HIV-1生命周期中直至并包括病毒融合的所有步骤。 它依赖于将β-内酰胺酶-Vpr(BlaM-Vpr)蛋白嵌合体并入病毒体中,并且随后通过融合将该嵌合体转移到靶细胞中(图1)。 通过CCF2(一种β-内酰胺酶的荧光染料底物)的酶裂解来监测转移,将其装载到靶细胞中。 β-内酰胺酶在CCF2中的β-内酰胺环的切割将染料的荧光发射光谱从绿色(520nm)改变为蓝色(447nm)。 这种变化反映了病毒体融合,并且可以通过流式细胞术检测(图2)。

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