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Author:
Date:
2014-07-05
[Abstract] Understanding the genetic safeguarding mechanism of Mycobacterium tuberculosis (Mtb) may help us to explain i), how Mtb survive the genetic assaults elicited by both reactive oxygen species (ROS) and reactive nitrogen species (RNS) produced by host macrophages and ii), why some strains of Mtb, e.g., Mtb strains from East Asian lineage and Beijing sublineage, exhibit high mutation rate and are more likely to acquire drug resistant mutations (e.g., rifampicin-resistance mutation) during infection. Mutation frequency analysis is a basic methods to study the genetic ...
[摘要] 了解结核分枝杆菌（Mtb）的遗传保护机制可以帮助我们解释i）如何Mtb存活由活性氧（ROS）和活性氮（RNS）产生的遗传攻击 宿主巨噬细胞和ii），为什么一些来自东亚谱系和北京亚系的Mtb菌株（例如eg）具有高突变率，并且更可能获得耐药突变（例如，利福平 - 抗性突变）。 突变频率分析是研究遗传保护机制的基本方法。 此外，为了研究突变的分子机制，有必要分析突变谱（例如，氧化的胞嘧啶可能诱导CG到TA突变）。 该协议描述了确定耻骨分枝杆菌（Msm）和Mtb中的突变频率和了解突变谱的方法。
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