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Author:
Date:
2011-10-05
[Abstract] Glutation S-transferase (GST) tagging is the most commonly used purification strategy for recombinant protein. It was developed with the goal of preserving the enzymatic activity by utilizing gentle elution condition of the target protein from purification matrix (Poon and Hunt., 1994). The method described here can be applied from single protein to proteome scale purification of recombinant protein from yeast (Zhu et al., 2000; Zhu et al., 2001).
[摘要] 谷胱甘肽S 转移酶（GST）标记是最常用的重组蛋白的纯化方法。通过利用温和的洗脱条件以保护酶的活性，使靶蛋白高纯化(1)。这里介绍的方法可以适用于从单一的蛋白质，以及蛋白质组大规模纯化重组蛋白酵母（2,3）。
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