| Polysome Fractionation to Analyze mRNA Distribution Profiles
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Author:
Date:
2017-02-05
[Abstract] Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the transcriptional and post-transcriptional levels. Among the latter steps, the regulation of translation is particularly important in cellular processes that require rapid changes in protein expression patterns. The translational efficiency of mRNAs is altered by RNA-binding proteins (RBPs) and noncoding (nc)RNAs such as microRNAs (Panda et al., 2014a and 2014b; Abdelmohsen et al., 2014). ...
[摘要] 真核细胞通过精确调节特定基因产物的表达来适应外部或内部信号的变化。蛋白质编码基因的表达受到转录和转录后水平的控制。在后面的步骤中，翻译的调节在需要蛋白质表达模式快速变化的细胞过程中特别重要。 mRNA的翻译效率由RNA结合蛋白（RBP）和非编码（nc）RNA如微RNA（Panda等人，2014a和2014b; Abdelmohsen等人）改变2014）。调节选择性mRNA翻译的因素的影响是RNA生物学中的一个关键问题。多核糖体（多核糖体）分馏分析是评估核糖体与给定mRNA的关联的有效方法。它提供了关于该mRNA的翻译状态的有价值的信息，这取决于与它们相关联的核糖体的数目，并且鉴定未翻译的mRNA（Panda等人，2016）。与许多核糖体相关的mRNA形成大量的多核糖体，预计将被主动翻译，而与少数或没有核糖体相关的mRNA有可能翻译不佳。总之，多聚糖分馏分析允许直接测定整个转录组水平的翻译效率以及个体mRNA。
背景 ...
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| Generation of Mouse Thyroid Calcitonin-producing Cell Tumors from Primary Mouse Tumors
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Author:
Date:
2015-12-20
[Abstract] Medullary thyroid cancers (MTCs) are derived from calcitonin-producing cells (C cells) of neuroendocrine origin. Rb heterozygous mice develop low-grade C cell adenocarcinoma following biallelic inactivation of the Rb tumor suppressor gene loci. Additional inactivation of another tumor suppressor gene such as Trp53, Arf or Cdkn1a allows Rb-deficient mice to generate more aggressive C cell adenocarcinoma (Takahashi et al., 2006; Shamma et al., 2009; Kitajima et al., 2015). To characterize C cell adenocarcinoma cells derived ...
[摘要] 甲状腺髓样癌（MTC）源自神经内分泌起源的降钙素产生细胞（C细胞）。Rb 杂合小鼠在b 肿瘤抑制基因位点的双等位基因失活后发展为低级C细胞腺癌。另一种肿瘤抑制基因（例如Trp53 ，Arf 或 Cdkn1a）的额外失活使Rb缺陷型小鼠产生更具侵袭性的C细胞腺癌（Takahashi， 2006; Shamma等人，2009; Kitajima等人，2015年）。为了表征来自不同遗传背景的Rb缺陷小鼠的C细胞腺癌细胞，我们尝试从原发性甲状腺肿瘤组织中提取C细胞腺癌细胞。由于原代小鼠小细胞肺癌（SCLC）细胞起源于也干细胞C的神经内分泌细胞，因此可以建立为非粘附和粘附细胞（Calbo等人，2011），我们将其方法应用于MTC。在这里我们描述我们的隔离技术的非粘附和粘附细胞培养从原发性甲状腺髓样肿瘤组织。我们发现C细胞的分子标记物如降钙素和Ascl1主要富集在非粘附性群体中（Kitajima等人，2015）。这与以下事实一致：最常分布的人MTC细胞系TT之一是非粘附性的。
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| Process and Analysis of Kidney Infiltrates by Flow Cytometry from Murine Lupus Nephritis
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Author:
Date:
2012-05-05
[Abstract] Methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE are essential to understand the patho-physiology of the disease. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans.An analysis of the function of these cells should lead to a better understanding of the inflammatory processes that lead to renal impairment in SLE and other renal inflammatory diseases.
[摘要] 分离和鉴定SLE（系统性红斑狼疮）小鼠肾脏单核吞噬细胞是了解疾病的病理生理必不可少的方法。这些细胞的激活与小鼠临床疾病的发病相关，并且这些细胞的浸润和人类疾病的预后不良相关。能够了解这些细胞的功能状态，就能更深入的了解SLE中导致肾功能不全和其他肾炎性疾病的炎症过程。
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