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Sodium pyrophosphate tetrabasic decahydrate

焦磷酸四钠十水合物

公司名称: Sigma-Aldrich
产品编号: S9515
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Biotinylation of Cell Surface Proteins
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Date:
2012-05-05
[Abstract]  Membrane proteins are major sensors of extracellular stimuli and initiators of intracellular signal transduction, and their abundance on the cell surface in particular is often dynamically regulated even when there are no significant changes of their total abundance in a cell. This protocol is designed to biochemically label and separate membrane proteins on the plasma membrane from those in the intracellular compartments. In conjunction with co-immunoprecipitation and western blot analysis, functional analysis of dynamic interaction of membrane proteins with other membrane proteins or ... [摘要]  膜蛋白是细胞外的刺激和细胞内信号转导发起的主要传感器,他们大量并特定的存在细胞表面上。它们虽然受到动态调节,但是在细胞中的总量没有显著变化。该方法的目的是给浆膜的膜蛋白打上生化标签,并且在细胞内将它们分离出来。与免疫共沉淀和免疫印迹分析相结合,膜蛋白和其他膜蛋白或者细胞内接头和效应蛋白的动态交互功能分析,都可以实现。

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