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Quick Spin Columns for radiolabeled RNA purification

快速旋转柱用于放射性标记的RNA纯化

公司名称: Roche Diagnostics
产品编号: 11274015001
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Dig RNA Probe Synthesis and Purification
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2012-01-20
[Abstract]  In situ hybridization is an effective method to examine the expression level and location of a gene of interest in tissues or cells. To do this, RNA can be labeled with digoxigenin-UTP (DIG) by in vitro transcription with SP6 and T7 RNA polymerase. The method provided in this protocol is a detailed description of synthesizing an antisense DIG-labeled RNA probe for in situ hybridization using the DIG RNA labeling kit from Roche. [摘要]  原位杂交技术是检查组织或细胞中基因表达水平和位置的一种有效的方法。在这个程序中提供的方法详细描述了一种合成反义探针的原位杂交。

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