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Author:
Date:
2012-05-20
[Abstract] Scientists commonly study the development of lymphocytes in two ways, adoptive transfer of hematopoietic stem cells or progenitor cells into recipient mice and fetal thymic organ culture (FTOC). Both strategies, especially the first one, are still widely used. However there are some limitations of these two methods such as being time consuming, resulting in limited cell yield, and challenges in the technology. During the last decade, OP9 stromal cells co-culture system has been modified to support lymphocyte development in vitro. This alternative way offers researchers a simple, ...
[摘要] 很长时间，科学家们用两种方法研究淋巴细胞的发育，造血干细胞的继承性转移及祖细胞到接受小鼠和胚胎胸腺器官培养(FTOC)。两种策略，尤其是第一种，现在仍然广泛使用。然而，这两种方法都有局限性，例如费时间，细胞产出有限和技术的挑战。过去的十几年，OP9间质细胞共培养系统被修饰用来在体外支持淋巴细胞发育。这是另外一种给研究者提供简单、有效在体外支持淋巴细胞祖先发育。这个系统更多重要的优点是很多因子参与到淋巴细胞的发育中，比如细胞因子类和Notch 信号途径，可以为了更深入的理解机制而被操作。这个方法是根据支持通过OP9-DL1间质细胞支持T细胞的经验。事实上，这个系统可以用于体外其他淋巴细胞的发育，例如B细胞核NK细胞,根据间质细胞的类型和不同细胞因子类的组合。
间质细胞，是从op/op小鼠骨髓中得到的，对巨噬细胞集落刺激因子(M-CSF)缺陷，为了在体外研究Notch 信号通路在T细胞分化过程，最近很多研究室通过共培养系统，包括在Notch配体中表达的as Delta-like 1, Delta-like 4或控制受体的淋巴祖细胞和修饰的OP9间质细胞。These derived OP9 stromal cells lines are termed as OP9-DL1, OP9-DL4, and OP9-V or OP9-vector, ...
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