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Boric acid

硼酸

公司名称: Sigma-Aldrich
产品编号: B0252
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Hippocampal Neuron Dissociation Transfection and Culture in Microfluidics Chambers
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2012-07-20
[Abstract]  Microfluidics chamber is an ideal tool to study local events that occurring in neuronal projections by perfectly compartmentalizing the cell soma from certain branches. It is very well suited for live cell imaging or immunohistochemistry staining. This protocol has been carefully modified in detail to fit the requirement of primary rat hippocampal neuronal cultures. It can also be applied to a more general neuronal culture purpose in microfluidics. [摘要]  微腔能够对某一分支的细胞进行很好地区分,因此它是研究发生在神经系统局部活动的游离工具,而且它特别适用于活细胞成像或者免疫组化印记。以下实验方案在细节方面进行了仔细的修改以便适用于小鼠海马体神经的培养,当然它也能适用于其它普通神经细胞在微腔中的培养。

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