| Cell Fractionation of Pseudomonas aeruginosa
|
|
Author:
Date:
2013-10-05
[Abstract] Pseudomonas aeruginosa is a Gram negative bacterium. Separating the cell envelope compartments enables proteins to be localized to confirm where in the cell they function. Cell fractionation can also provide a first step in a protein purification strategy (Williams et al., 1998). This protocol has been designed to obtain the different fractions of P. aeruginosa, namely the inner membrane, outer membrane, cytoplasmic and periplasmic compartments. Specific detection of the arginine specific autotransporter (AaaA) (Luckett et al., 2012) in the outer membrane ...
[摘要] 铜绿假单胞菌是革兰氏阴性细菌。 分离细胞包膜区室使得蛋白质能够被定位以确认它们在细胞中的何处起作用。 细胞分级还可以提供蛋白质纯化策略的第一步（Williams等人，1998）。 该方案已经设计成获得不同比例的P。 铜绿假单胞菌，即内膜，外膜，细胞质和周质区室。 在P的外膜中特异性检测精氨酸特异性自转运体（AaaA）（Luckett等人，2012）。 已经使用该协议进行了铜绿假单胞菌。
|
|
|
| Isolation of Inner Membrane Vesicles from Escherichia coli by Using an Affinity Tag
|
|
Author:
Date:
2012-10-20
[Abstract] This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity capture. The specificity of the polar vesicle isolation was confirmed by mass spectrometry that identified more than one hundred proteins, most of which are known inner membrane proteins, including other known polar proteins. This protocol, or if adapted properly by choosing other affinity targets, is well suited to isolate other ...
[摘要] 该实验方案的中文版正在准备中．．．
|
|
|