Deoxyribonuclease I

脱氧核糖核酸酶I

公司名称: MP Biomedicals

产品编号: 02100575

Preparation and Purification of β-1,3-glucan-Linked Candida glabrata Cell Wall Proteases by Ion-Exchange Chromatography, Gel Filtration, and MDPF-Gelatin-Zymography Assay

Pirjo Pärnänen, Timo Sorsa, Taina Tervahartiala and Pirjo Nikula-Ijäs

Published online: 2024-03-20

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Isolation of Inner Membrane Vesicles from Escherichia coli by Using an Affinity Tag

Author:

Gang Li and Kevin D. Young,

Date:

2012-10-20

[Abstract] This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity capture. The specificity of the polar vesicle isolation was confirmed by mass spectrometry that identified more than one hundred proteins, most of which are known inner membrane proteins, including other known polar proteins. This protocol, or if adapted properly by choosing other affinity targets, is well suited to isolate other ... [摘要] 该实验方案的中文版正在准备中．．．

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Deoxyribonuclease I

脱氧核糖核酸酶I

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