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PIPETMAN Neo®, P10N, 1-10µL

公司名称: Gilson
产品编号: F144561
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Bacterial Aggregation Assay in the Presence of Cyclic Lipopeptides
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2018-01-05
[Abstract]  Lipopeptides is an important class of biosurfactants having antimicrobial and anti-adhesive activity against pathogenic bacteria. These include surfactin, fengycin, iturin, bacillomycin, mycosubtilin, lichenysin, and pumilacidin (Arima et al., 1968; Naruse et al., 1990; Yakimov et al., 1995; Steller and Vater, 2000; Roongsawang et al., 2002; Vater et al., 2002). To date, none of these lipopeptides have been reported to possess any anti-motility activity. We isolated, purified and characterized two novel cyclic lipopeptides (CLPs) from Bacillus ... [摘要]  脂肽是一类重要的生物表面活性剂,对致病菌具有抗菌和抗粘连活性。这些包括表面活性肽,fengycin,伊库菌素,杆菌霉素,mycosubtilin,地衣素和pumilacidin(Arima等人,1968; Naruse等人,1990; Yakimov等人1995; Steller和Vater,2000; Roongsawang等人,2002; Vater等人,2002)。迄今为止,这些脂肽都没有被报道具有任何抗运动活性。我们从芽孢杆菌分离,纯化和鉴定了两种新的环状脂肽(CLPs)。 176使用高效液相色谱,质谱和核磁共振光谱。 CLPs极大地抑制致病性溶藻弧菌的运动,并促进细胞聚集而不诱导细胞死亡。根据Dalili所述用于抗生物膜测定的方法进行细胞聚集测定(Dalili等人,2015)。将来,该测定法可以适用于测试来自细菌的脂肽样活性物质的细胞聚集和抗生物膜活性。

【背景】过度使用广谱抗生素和随之而来的抗药性细菌的增殖刺激了开发环境友好型生物防治措施的努力,以减少健康危害和环境污染(Nam等人,2016; Sajitha >等。,2016)。近年来,生物表面活性剂的抗微生物特性已被越来越多地用于抗菌,抗真菌和抗病毒的应用(Cameotra和Makkar,2004; Singh和Cameotra,2004; Rodrigues等人 ...

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