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公司名称: Tarson
产品编号: 500020
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In vivo Quantification of Alkanes in Escherichia coli
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Date:
2020-04-20
[Abstract]  Microbial production of alkanes employing synthetic biology tools has gained tremendous attention owing to the high energy density and similarity of alkanes to existing petroleum fuels. One of the most commonly studied pathways includes the production of alkanes by AAR (acyl-ACP (acyl carrier protein) reductase)-ADO (aldehyde deformylating oxygenase) pathway. Here, the intermediates of fatty acid synthesis pathway are used as substrate by the AAR enzyme to make fatty aldehyde, which is then deformylated by ADO to make linear chain alkane. However, the variation in substrate availability to ... [摘要]  [摘要] 由于烷烃的高能量密度和与现有石油燃料的相似性,使用合成生物学工具生产烷烃的微生物受到了广泛关注。最常研究的途径之一是通过AAR(酰基-ACP (酰基)载体蛋白)还原酶)-ADO(醛Deformylating 加氧酶)途径。在这里,中间体脂肪酸合成途径被用作基材由AAR Enzym E要使脂肪醛,然后是Deformylated 通过ADO,使线性链烷烃。但是,即该途径的第一种酶的底物利用率的变化,即,AAR,通过脂肪酸合成途径和ADO酶的低周转率,使得在体内条件下的产量和效价的计算极为困难。在体内测定中,将确定的ADO酶底物外加到培养基中有助于监测菌体的流入。因此,该底物提供了更准确的产物收率测量方法。在此方案中,我们包括用于实施体内测定法以监测大肠杆菌中烷烃生产的详细指南。

[背景] 利用工程微生物生产烷烃的研究已广受欢迎,因为它提供了一种有吸引力的替代方案,可减少对化石燃料的依赖,同时减轻气候变化的影响(Lee 等,2008;Knothe ,2010; Lu,2010; Schirmer 等。人,2010;谭等人。,2011)各种途径已被发现或人工Assemb ...

Bacteria-fungal Confrontation and Fungal Growth Prevention Assay
Author:
Date:
2018-01-20
[Abstract]  There are some bacteria which can grow and multiply at the cost of living fungal biomass. They can potentially utilize fungi as a source of nutrients to forage over them. Such phenomenon is known as bacterial mycophagy, however, its mechanistic insights need to be explored to identify the molecules involved in mycophagy for potential utilization in controlling various fungal diseases. Recently we have demonstrated that a rice-associated bacteria Burkholderia gladioli strain NGJ1 exhibits mycophagous ability on several fungi, including Rhizoctonia solani, the necrotrophic ... [摘要]  有一些细菌可以生长和繁殖以牺牲真菌生物量为代价。 他们可能潜在地利用真菌作为营养素的来源来搜寻它们。 这种现象被称为细菌真菌病,然而,其机理性的见解需要被探索,以确定参与mycophagy分子潜在的利用控制各种真菌病害。 最近我们已经证明,稻米相关的细菌伯克霍尔德氏唐菖蒲菌株NGJ1在几种真菌上展现出真菌的能力,包括水稻纹枯病真菌病原体,即立枯丝核菌(Rhizoctonia solani)。 我们在此描述我们用于研究B的已验证的和有效的方法。 剑兰菌株NGJ1- R。 solani 互动。 这些方法学可用于设计分析来研究细菌和真菌之间的对抗,从而能够从这些细菌中发现新的抗真菌分子。

【背景】立枯丝核菌(Rhizoctonia solani)是具有多种宿主的重要的植物病原真菌。导致稻纹枯病;在爆发性疾病之后,稻米的第二大破坏性真菌病(Fisher等人,2012; Ghosh等人,2014; Ghosh等人, >,2017)。在发展水稻纹枯病防治方法之后,我们从水稻幼苗中分离出一株具有广谱抗真菌活性的细菌。基于rDNA和基因组测序,该细菌已经被鉴定为唐菖蒲伯克霍尔德氏菌菌株NGJ1(Jha等人,2015)。除了抗真菌特性外,我们观察到NGJ1具有对真菌进行觅食的能力,并且在禾本科植物上表现出食菌能力。 solani 以及其他各种真菌。在与 R交互之后。 ...

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