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Gastight syringe for GC

公司名称: Hamilton
产品编号: 1701 SN CTC
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Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining
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2018-03-05
[Abstract]  Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas chromatography. Our first method describes how to analyze PHB granules by fluorescence microscopy qualitatively. Our second approach enables the conversion of PHB to volatile hydroxycarboxylic acid methyl esters by acidic methanolysis and their quantification by gas chromatography. Through this method, it is possible to obtain an absolute ... [摘要]  在生长过程中,富养罗尔斯通氏菌H16产生和动员(重新利用)细胞内聚羟基丁酸酯(PHB)颗粒。 该协议描述了细胞内尼罗红染色的PHB颗粒的可视化和通过气相色谱定量PHB。 我们的第一种方法描述了如何通过荧光显微镜定性分析PHB颗粒。 我们的第二种方法可以通过酸性甲醇分解和气相色谱定量法将PHB转化为挥发性羟基羧酸甲酯。 通过该方法,可以获得PHB的绝对定量,例如,每细胞干重。

【背景】聚羟基脂肪酸酯(PHA),尤其是聚羟基丁酸酯(PHB),是许多原核生物物种中的能量和碳储存化合物,确保细菌在压力条件下存活(Anderson和Dawes,1990;Pötter和Steinbüchel,2006; Jendrossek和Pfeiffer,2014; Bresan, >等。,2016)。这些生物聚合物的工业应用是生物降解塑料的生产(Chen,2009; Riedel等人,2015)和潜在药物成分的研究(Wu,2009; Zonari等人, ,2015; Pacheco et al。,2015; Giretova ...

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