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Author:
Date:
2013-03-20
[Abstract] Natural killer T (NKT) cells comprise an important immunoregulatory T cell subset and express cell surface proteins characteristic of both natural killer cells and T cells. Invariant NKT (iNKT) cells are activated by lipid antigen presented in the context of CD1d molecules, in contrast to classic T cell subsets which recognize peptide antigens presented by MHC molecules. Following activation, iNKT cells rapidly secrete large amounts of cytokines and can lyse tumor cells and virally infected cells; however, iNKT cells are reduced in patients with autoimmune disease and cancer. The potential ...
[摘要] 自然杀伤T（NKT）细胞包含重要的免疫调节T细胞亚群，并表达天然杀伤细胞和T细胞特有的细胞表面蛋白。与识别由MHC分子呈递的肽抗原的经典T细胞亚群形成对比，不变NKT（iNKT）细胞被在CD1d分子的环境中存在的脂质抗原活化。激活后，iNKT细胞快速分泌大量细胞因子，并且可以裂解肿瘤细胞和病毒感染的细胞;然而，iNKT细胞在患有自身免疫性疾病和癌症的患者中减少。表征和研究iNKT细胞用于治疗目的的前瞻性用途的潜力随着刺激和扩增人iNKT细胞的能力而显着增加。在这个协议，我们描述一种生成和传播原代人类iNKT细胞的方法。具体地，从人外周血单核细胞（PBMC）分离初级iNKT细胞，然后在人IL-2存在下用照射的α-GalCer负载的自体未成熟树突细胞（DC）定期扩增。
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