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Lipofectamine® 2000 Transfection Reagent

Lipofectamine ® 2000转染试剂

公司名称: Thermo Fisher Scientific
产品编号: 11668
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Test MicroRNA Target with in vitro Cell Culture Luciferase Assay
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2013-03-05
[Abstract]  MicroRNAs (miRNAs) are small non-coding RNAs of 21-24 nucleotides in length that modulate gene expression by targeting the untranslated region (UTR) of mRNA. This protocol is to be used to test the binding and activity of miRNA on putative UTR target sequences. It is based on the expression of Luciferase as a reporter gene fused to the UTR sequence in the presence of plasmids containing pre-miRNA of interest or synthetic miRNA to test in an in vitro cell culture assay. [摘要]  微小RNA(miRNA)是长度为21-24个核苷酸的小非编码RNA,通过靶向mRNA的非翻译区(UTR)来调节基因表达。 该方案用于测试miRNA对推定的UTR靶序列的结合和活性。 它是基于荧光素酶作为报道基因的表达,其在含有目的前miRNA或合成miRNA的质粒存在下在UTR体外融合,以在体外细胞培养测定中测试。

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