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Mouse IFN-γ ELISpot BASIC (ALP)

小鼠IFN-γELISpot基础(ALP)

公司名称: Mabtech
产品编号: 3321-2A
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INF-gamma Release ELISpot Assay
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2012-03-20
[Abstract]  IFN-γ (Interferon-gamma) is produced mainly by activated T cells and NK cells. Production of IFN-γ (Interferon-gamma) by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ (Interferon-gamma) is typically associated with effective host defense against intracellular pathogens. The Enzyme-Linked ImmunoSpot (ELISpot) assay is commonly used to assess the function of antigen specific T cells by detecting IFN-γ release. The ELISpot assay is a very sensitive immunoassay, allowing the detection of a secreted cytokine at the single ... [摘要]  IFN-γ(干扰素-γ)主要产生于激活的T细胞和NK细胞。TH1型响应的特点是通过辅助T细胞和细胞毒T细胞产生IFN-γ,所以高水平IFN-γ的产生是由于寄主应对细胞内的病原体产生了有效的防御。酶联免疫斑点法通常用于评估抗原特异的T细胞的功效,即检测IFN-γ的释放。酶联免疫斑点法是一种非常灵敏的免疫检测方法,它可以在单细胞水平检测细胞因子的分泌。酶联免疫斑点法是最灵敏的细胞检测方法之一,它检测的灵敏度可以达到1:100000个细胞。依赖于化学物质的分析,酶联免疫斑点分析可以比传统的ELISA灵敏20到200倍。

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