| Maize Embryo Transient Transformation by Particle Bombardment
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Author:
Date:
2013-08-20
[Abstract] Particle bombardment has been shown to be a useful method to study gene promoter regulatory elements by transient transformation of maize embryos with different constructions of gene promoters fused to a gene reporter. DNA to transfer is coated to high density gold microparticles and introduced into cells when accelerated by a helium pulse. This method allows a first rapid approach, avoiding time consuming stable transformation of maize plants and also allows quantitative promoter expression analysis by a histochemical or fluorometric assay.
[摘要] 已经证明粒子轰击是通过与基因报告基因融合的不同构建的基因启动子的玉米胚的瞬时转化来研究基因启动子调节元件的有用方法。 转移的DNA被涂覆到高密度的金微粒上，并通过氦脉冲加速时被引入细胞。 该方法允许第一快速方法，避免耗时的玉米植物的稳定转化，并且还允许通过组织化学或荧光测定进行定量启动子表达分析。
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| Modified Single-Cell Transient Gene Expression Assay in Barley Epidermal Cells
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Author:
Date:
2013-05-05
[Abstract] Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow gun of the model PDS-1000/He system (Bio-Rad). This assay was originally optimized for analyzing cell death activity and disease resistance function of the barley MLA (mildew locus A) disease resistance proteins against the powdery mildew fungus, which can be further adopted for other purposes for other types of plant proteins and in some other ...
[摘要] 通过生物射弹颗粒递送的瞬时基因表达是广泛使用的用于植物中基因功能分析的技术。 在该协议中，我们描述了通过用PDS-1000/He系统（Bio-Rad）的粒子流入枪转化的修饰的单细胞瞬时表达测定。 该测定最初被优化用于分析大麦MLA（霉菌基因座A）疾病抗性蛋白质对白粉菌真菌的细胞死亡活性和疾病抗性功能，其可以进一步用于其他目的用于其他类型的植物蛋白质和其他一些 植物物种，包括拟南芥（Arabidopsis thaliana）。
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