| Isolation and in vitro Activation of Mouse Peyer’s Patch Cells from Small Intestine Tissue
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Author:
Date:
2014-11-05
[Abstract] The lumen of gastrointestinal tract is exposed to several potentially pathogenic microorganisms, thus it is extremely relevant to understand how immunosurveilance can be established. Peyer’s Patches (PPs) are oval or round lymphoid nodules that protrude from the outer wall of the ileum portion of small intestine. PPs contain a high percentage of B and T lymphocytes, macrophages and dendritic cells. Here we summarize a protocol for isolation and culture of mouse PP cells, which can be used to get a better insight into immunopathologies of microbes and to evaluate immune responses elicited by ...
[摘要] 胃肠道的腔暴露于几种潜在的致病微生物，因此，了解如何建立免疫耐受是非常重要的。 派尔氏贴剂（PP）是椭圆形或圆形淋巴结，其从小肠的回肠部分的外壁突出。 PP含有高百分比的B和T淋巴细胞，巨噬细胞和树突细胞。 在这里我们总结一个协议的小鼠PP细胞的分离和培养，可用于更好地了解微生物的免疫病理学和评估由粘膜疫苗引发的免疫反应。
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| Identification of Helminth-induced Type 2 CD4+ T Cells and ILC2s
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Author:
Date:
2014-06-05
[Abstract] After activation, T cells differentiate into different T helper (Th) subsets, namely Th1, Th2, and Th17. These different Th subsets are associated with the production of particular cytokines endowing them with different functions. In immunity against helminth infections the Th2 cell subset plays an important role. Th2 cells typically produce IL-4, IL-5, IL-13, and IL-9 resulting in antibody-isotype switching to IgE, eosinophilia, basophilia, mucin production, and smooth muscle cell hyperactivity. Here we analyze the development of the pathogen specific Th2 immune responses in mice after ...
[摘要] 活化后，T细胞分化成不同的T辅助（Th）子集，即Th1，Th2和Th17。 这些不同的Th亚群与赋予它们不同功能的特定细胞因子的产生相关。 在抵抗蠕虫感染的免疫中，Th2细胞亚群起重要作用。 Th2细胞通常产生IL-4，IL-5，IL-13和IL-9，导致抗体同种型转换为IgE，嗜酸性粒细胞增多，嗜碱性粒细胞，粘蛋白产生和平滑肌细胞多动。 在这里我们分析感染后的蠕虫寄生虫，Heligmosomoides polygyrus bakeri，诱导的先天淋巴细胞2型（ILC2）和炎症小体的激活的病原体特异性Th2免疫反应的发展 巨噬细胞通过 Heligmosomoides polygyrus bakeri的排泄/分泌产物。
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| Intracellular Cytokine (INF-gamma) Staining Assay
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Author:
Date:
2012-04-05
[Abstract] An intracellular cytokine (INF-gamma) staining assay is used to analyze the function of lymphocytes at the single cell level. By combining surface staining and intracellular cytokine staining, this assay can reveal the percentage of cytokine-releasing cells in a particular population, which cannot be obtained from an ELISpot assay.
[摘要] 胞内细胞因子(INF-gamma)染色方法是在单细胞水平分析淋巴细胞的功能。通过结合表面染色和细胞内细胞因子染色，细胞内细胞因子（干扰素-γ）染色法是用来分析在单细胞水平淋巴细胞的功能。通过结合表面染色和细胞内细胞因子染色，此法可以获得在一定数量群体中，能够释放细胞因子细胞的百分比，而这些是无法用酶联免疫斑点法检测方法所获得的。
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