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Dithiothreitol

二硫苏糖醇

公司名称: Affymetrix
产品编号: 15397
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GTP Binding Assays in Arabidopsis thaliana
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2013-05-05
[Abstract]  Signaling through G proteins constitutes an ancient mechanism that functions in the transduction of extracellular signals into intracellular responses. Activation of a proper receptor by a stimuli leads to an exchange of GDP for GTP, the activation of G proteins and the dissociation of Gα-GTP from the Gβγ dimer for the heterotrimeric G proteins. The G protein subunits remain active until the intrinsic GTPase activity or/and a GTPase activating protein result in the hydrolysis of GTP to GDP and the inactivation of the protein. Here we describe a protocol for measuring GTP ... [摘要]  通过G蛋白的信号传导构成了在将细胞外信号转导到细胞内反应中起作用的古老机制。通过刺激激活适当的受体导致GTP的GDP交换,G蛋白的激活和G emα-GTP从G emβγ二聚体的解离异源三聚体G蛋白。 G蛋白亚基保持活性,直到内在的GTP酶活性或/和GTP酶活化蛋白导致GTP水解为GDP和蛋白质的失活。在这里,我们描述了使用GTPγ S,从拟南芥植物蛋白提取物测量GTP结合活性的方案。 GTPγ测定通过测定不可水解的类似物GTPγ35 S的结合活性来测量刺激后G蛋白活化的水平。为了确定特异性G蛋白活性,应包括特异性突变体和/或过表达体提取物,并作为对照测量。

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