| Generation of Polyclonal Specific Antibodies
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Author:
Date:
2013-06-05
[Abstract] Generation of antibodies specific for a protein of interest is a common method in many disciplines. This protocol details the steps in production of a polyclonal antibody in rabbits using a bacterially expressed fusion protein as an antigen. The protocol is generated based on data presented in Wirschell et al.(2013).
[摘要] 对感兴趣的蛋白质特异性的抗体的产生是许多学科中的常见方法。 该方案详述了使用细菌表达的融合蛋白作为抗原在兔中产生多克隆抗体的步骤。 该协议基于Wirschell等人（2013）中提供的数据生成。
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| Immunofluorescence (Especially for Cells Growing on a Coverglass)
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Author:
Date:
2012-02-20
[Abstract] If an antibody for your protein of interest is available, immunofluorscence is a useful method to detect the localization and relative abundance of the protein by using a fluorescence microscope. Immunofluoresence can be used in combination with other, non-antibody methods of fluorescence staining, for example, the use of DAPI to label DNA. This protocol describes setting up an immunofluorescence experiment using cells grown on a coverglass.
[摘要] 如果你有你所感兴趣的蛋白的抗体，通过使用荧光显微镜检测蛋白定位和相关蛋白丰度的免疫荧光法是一个好方法。使用免疫荧光法的同时能够结合其它的，非抗体荧光染色方法，如使用DAPI标记DNA。
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| Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
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Author:
Date:
2012-02-05
[Abstract] Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively replicating their DNA and estimating for the percentage of cells in S-phase. The immunocytochemical detection of BrdU incorporated into DNA is a powerful tool to study the cytokinetics of normal and tumor cells. In vitro or in vivo labeling of tumor cells with BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU ...
[摘要] 溴脱氧尿苷(BrdU) 是一种胸苷类似物并且可以掺入心合成的S期细胞的DNA。BrdU 特异性抗体可以用于检测掺入的BrdU，从而可以表明细胞复制DNA和检测在S期细胞的比例。用免疫细胞化学检测掺入DNA中的BrdU是一种很有力的研究普通或者癌症细胞动力学的工具。用BrdU在体内或者体外标记癌细胞和接下来用BrdU特性性抗体检测掺入的BrdU是一种精确的应用广泛的方法来定量DNA的合成程度。
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