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di-Potassium Hydrogen Phosphate anhydrous

磷酸氢二钾

公司名称: AppliChem
产品编号: A1042
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Detection of ASC Oligomerization by Western Blotting
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2017-05-20
[Abstract]  The apoptosis-associated speck-like protein with a caspase-recruitment domain (ASC) adaptor protein bridges inflammasome sensors and caspase-1. Upon inflammasome activation, ASC nucleates in a prion-like manner into a large and single platform responsible for the recruitment and the activation of caspase-1. Active caspase-1 will in turn promote the proteolytic maturation of the pro-inflammatory cytokine IL-1β. ASC oligomerization is direct evidence for inflammasome activation and its detection allows a read-out independent of caspase-1 and IL-1β. This protocol describes how to detect the ... [摘要]  具有半胱天冬酶募集区(ASC)衔接蛋白的凋亡相关斑点样蛋白桥联炎症体传感器和半胱天冬酶-1。在炎性体活化后,ASC以类似朊病毒的方式成核,成为负责募集和激活半胱天冬酶-1的大而单一的平台。活性胱天蛋白酶-1将反过来促进促炎细胞因子IL-1β的蛋白水解成熟。 ASC寡聚化是炎性体激活的直接证据,其检测允许读取与caspase-1和IL-1β无关。该方案描述了如何通过蛋白质印迹检测ASC的寡聚化。

背景 Inflammasomes是大量的多蛋白平台,其感测各种微生物,内源和环境胁迫因子,导致促炎IL-1细胞因子家族的成熟(Martinon等人,2002; Sharma和Kanneganti, 2016)。激活后,炎性细胞传感器通过pyrin结构域(PYD)-PYD同型相互作用募集衔接蛋白ASC。 ASC通过胱天蛋白酶激活和募集域(CARD)-CARD相互作用又结合半胱天冬酶-1,并有利于caspase-1的自我蛋白水解切割,导致IL-1β和IL-18的成熟(Hoss等人。,2016)。 Inflammasome激活引发ASC二聚体的超分子寡聚化成称为“ASC-specks”或“pyroptosome”(Fernandes-Alnemri等人,2007)的大交织原纤维。 ASC-speck / ...

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