{{'Search' | translate}}
 

Whatman® Protran® nitrocellulose membranes

Whatman ® Protran ®硝化纤维素膜

公司名称: Sigma-Aldrich
产品编号: Z670634
Bio-protocol()
Company-protocol()
Other protocol()

Amino Acid Transport Assays in Resting Cells of Lactococcus lactis
Author:
Date:
2013-06-20
[Abstract]  Many bacteria are auxotrophic for at least a number of amino acids for which they lack the biosynthetic pathways. The organisms are still able to grow in media containing free amino acids as the sole source of amino acid when transport systems for the free amino acids are present in the cytoplasmic membrane. A range of transport systems for essential as well as non-essential amino acids has been described that use the proton motive force (by proton symport) or ATP hydrolysis as driving force to allow for the accumulation of the amino acid in the cells. The most widely used assay for uptake of ... [摘要]  许多细菌是至少一些氨基酸的营养缺陷型,它们缺少生物合成途径。 当游离氨基酸的运输系统存在于细胞质膜中时,生物体仍然能够在含有游离氨基酸作为唯一氨基酸来源的培养基中生长。 已经描述了使用质子动力(通过质子对数)或ATP水解作为驱动力以允许氨基酸在细胞中积累的必需氨基酸和非必需氨基酸的一系列运输系统。 用于摄取氨基酸(或任何其它底物)的最广泛使用的测定法是使用放射性标记底物的快速过滤测定法。 在这里,我们描述了由葡萄糖激发的乳酸乳球菌(乳酸乳球菌)的静息细胞中摄取的测定。

产品评论