| MNase Digestion for Nucleosome Mapping in Neurospora
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Author:
Date:
2016-06-05
[Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus Neurospora crassa chromatin without a requirement of a nuclear fractionation step.
[摘要] 通过微球菌核酸酶MNase消化染色质，然后高通量测序允许我们确定核小体在基因组上的位置和占据。 在这个协议中，我们描述了MNase消化丝状真菌粗糙链孢霉染色体的优化条件，而不需要核分馏步骤。
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| Isolation of Growth Cones from Mouse Brain
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Author:
Date:
2013-08-05
[Abstract] Growth cones are motile structures at the tips of growing neurites, which play an essential role in regulation of growth and navigation of growing axons and dendrites of neurons in the developing nervous system. This protocol describes isolation of growth cones from the brain tissue from young mice. Growth cones isolated using this protocol have been extensively characterized using electron microscopy (Pfenninger et al., 1983) and may be used for any kind of subsequent biochemical and/or functional analyses, including Western blot analysis of protein expression (Westphal et al ...
[摘要] 生长锥是生长神经突的尖端的运动结构，其在调节发育中的神经系统中生长的轴突和神经元的树突的生长和导航中发挥重要作用。 该方案描述了来自幼鼠的脑组织的生长锥的分离。 使用该方案分离的生长锥已经使用电子显微镜广泛地表征（Pfenninger等人，1983），并且可以用于任何种类的随后的生物化学和/或功能分析，包括蛋白质表达的蛋白质印迹分析（Westphal et al。 2010），分析生长锥积累酶的活性（Leshchyns'ka et al。，2003; Li et al。，2013），以及内吞作用和胞吐作用率的分析（Chernyshova等，2011）。
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| Isolation of Phagosomes from Dendritic Cells by Using Magnetic Beads
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Author:
Date:
2013-07-20
[Abstract] Phagosomes are intracellular organelles in dendritic cells in which pathogens such as viruses, bacteria and parasites are internalised to be proteolysed and killed. Phagosomes are formed by fusion with the plasma membrane, some area of the endoplasmic reticulum as well as the lysosome. This protocol described the purification of phagosomal compartments at different stage of their maturation using magnetic beads.
[摘要] 吞噬体是树突细胞中的细胞内细胞器，其中病原体例如病毒，细菌和寄生虫被内化以被蛋白水解和杀死。 吞噬体通过与质膜，内质网的一些区域以及溶酶体融合形成。 该协议描述了使用磁珠在其成熟的不同阶段的吞噬体隔室的纯化。
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