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WheatonTM Potter-Elvehjem Tissue Grinders

Wheaton TM Potter-Elvehjem 组织研磨机

公司名称: Thermo Fisher Scientific
产品编号: 08-414-14A
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Isolation of Growth Cones from Mouse Brain
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2013-08-05
[Abstract]  Growth cones are motile structures at the tips of growing neurites, which play an essential role in regulation of growth and navigation of growing axons and dendrites of neurons in the developing nervous system. This protocol describes isolation of growth cones from the brain tissue from young mice. Growth cones isolated using this protocol have been extensively characterized using electron microscopy (Pfenninger et al., 1983) and may be used for any kind of subsequent biochemical and/or functional analyses, including Western blot analysis of protein expression (Westphal et al ... [摘要]  生长锥是生长神经突的尖端的运动结构,其在调节发育中的神经系统中生长的轴突和神经元的树突的生长和导航中发挥重要作用。 该方案描述了来自幼鼠的脑组织的生长锥的分离。 使用该方案分离的生长锥已经使用电子显微镜广泛地表征(Pfenninger等人,1983),并且可以用于任何种类的随后的生物化学和/或功能分析,包括蛋白质表达的蛋白质印迹分析(Westphal et al。 2010),分析生长锥积累酶的活性(Leshchyns'ka et al。,2003; Li et al。,2013),以及内吞作用和胞吐作用率的分析(Chernyshova等,2011)。

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