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Author:
Date:
2013-08-20
[Abstract] Bronchoalveolar lavage (BAL) is a simple but valuable and typically performed technique commonly used for studying the pathogenesis of lung diseases such as asthma and COPD. Cell counts can be combined with new methods for examining inflammatory responses, such as ELISA, Flow cytometric analysis, immunohistochemistry, quantitative polymerase chain reaction, and HPLC to assess cellular expression for inflammatory cytokines and growth factor. Here we describe a basic procedure to collect BAL fluid and digest lung tissue for assessing a number of pulmonary components.
[摘要] 支气管肺泡灌洗（BAL）是一种简单但有价值且通常执行的技术，通常用于研究肺病如哮喘和COPD的发病机制。 细胞计数可以与用于检查炎症反应的新方法组合，例如ELISA，流式细胞术分析，免疫组织化学，定量聚合酶链反应和HPLC以评估炎症细胞因子和生长因子的细胞表达。 在这里，我们描述了收集BAL液和消化肺组织以评估肺部成分数量的基本程序
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