A 3D Culture System of Human Immortalized Myometrial Cells
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Author:
Date:
2016-10-20
[Abstract] Myometrium forms the middle layer of the uterus and is mainly composed of the smooth muscle cells. The cells in vitro are usually grown in a single layer (2-dimensional; 2D) format, whereas in vivo cells are structured in an extracellular matrix scaffolding that allows the cells to communicate and respond to environmental cues. We have developed human myometrium and leiomyoma 3-dimensional (3D) culture, wherein the cells retain their molecular characteristics and respond to environmental cues (Malik and Catherino, 2012; Malik et al., 2014).
[摘要] 子宫肌层形成子宫的中间层,主要由平滑肌细胞组成。细胞在体外通常生长在单层(2维; 2D)格式中,而体内细胞在细胞外基质支架中结构化,其允许细胞沟通和响应环境线索。我们已经开发了人子宫肌瘤和平滑肌瘤3维(3D)培养物,其中细胞保留其分子特征并响应环境线索(Malik和Catherino,2012; Malik等人,2014)。 br /> [背景] 在过去十年中,随着更多实验室从使用人工2D格式的细胞培养转移到3D细胞培养模型系统,观察到一定的转变,其中细胞生长在允许它们附着并获得更生理结构的基质中。该模型系统为细胞提供更自然的分化状态,并且培养的细胞在体内形成组织样环境。这是一个详细的协议为myometrium 3D细胞培养生长胶原-1矩阵,修改从Malik和Catherino(2012年)。
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Fluorescence in situ Hybridization to the Polytene Chromosomes of Anopheles Mosquitoes
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Author:
Date:
2013-08-20
[Abstract] Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This protocol was used to map genes (Sharakhova et al., 2010) and microsatellite markers (Kamali et al., 2011; Peery et al., 2011) on polytene chromosomes from ovarian nurse cells and salivary glands of malaria mosquitoes. Detailed physical genome mapping ...
[摘要] 荧光原位杂交(FISH)是一种使用荧光标记的DNA探针来绘制遗传元件在染色体上的位置的方法。 通过缺口翻译或随机引物标记法将Cy-3或Cy-5标记的核苷酸掺入DNA中来制备DNA探针。 该方案用于将基因(Sharakhova等人,2010)和微卫星标记物(Kamali等人,2011; Peery等人, >,2011)对来自卵巢保护细胞和疟疾蚊子的唾液腺的多角体染色体。 在多聚染色体上进行的详细的物理基因组作图具有将DNA序列连接到特异性染色体结构(例如异染色质)的潜力(Sharakhova等人,2010)。 该方法还允许比较细胞遗传学研究(Sharakhova等人,2011; Xia等人,2010)和物种系统发育的重建(Kamali等人, ,2012)。
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