|
Author:
Date:
2013-08-20
[Abstract] SVZ-derived progenitors grown as neurospheres is a well-known model to study neural stem cell and progenitor functions such as proliferation, differentiation/self-renewal, and migration (Durbec and Rougon, 2001). This protocol is for preparing a culture of SVZ-derived progenitors from 8 early postnatal mouse brains (P0 to P3). One week after cell plating, we can observe round floating neurospheres, each resulting from the clonal expansion of a single EGF/FGF responsive neural progenitor.
[摘要] 作为神经球生长的SVZ衍生的祖细胞是研究神经干细胞和祖细胞功能如增殖，分化/自我更新和迁移的众所周知的模型（Durbec和Rougon，2001）。 该方案用于从8只早期出生后的小鼠脑（P0至P3）制备SVZ衍生祖细胞的培养物。 细胞电镀后一周，我们可以观察到圆形浮游神经球，每个由单个EGF / FGF反应性神经祖细胞的克隆扩增产生。
|