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Author:
Date:
2013-09-05
[Abstract] We describe an efficient method to obtain a sufficient quantity of RNA from nematode-induced galls with a high quality and integrity, proved to be appropriate for transcriptomic analysis, i.e. real time PCR, microarray hybridization or second generation sequencing. This protocol is efficient for small quantities of galls (organs with high protein and sugar contents). The protocol allows obtaining an RNA yield of 5-15 μg total RNA from 250-300 hand dissected galls at 3 days post infection (dpi) (Figure 1). It was proved particularly for Arabidopsis and tomato.
[摘要] 我们描述了一种有效的方法，以高质量和完整性从线虫诱导的gall中获得足够量的RNA，证明适合于转录组分析，即实时PCR，微阵列杂交或第二代测序 。 该方案对于少量胆汁（具有高蛋白质和糖含量的器官）是有效的。 该协议允许在感染后3天（dpi）从250-300个手部解剖的疱疹获得5-15μg总RNA的RNA产量（图1）。 它被证明特别适用于拟南芥和番茄。
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