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SODIUM PHOSPHATE MONOBASIC

磷酸二氢钠

公司名称: BioShop Canada
产品编号: SPM400
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Quantification of Total and Soluble Inorganic Phosphate
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Date:
2013-09-05
[Abstract]  A simple, rapid, and sensitive colorimetric microassay for inorganic phosphate (Pi) relies upon the absorption at 660 nm of a molybdenum blue complex that forms upon reduction of an ammonium molybdate-Pi complex in acid. The method for determination of total Pi uses plant tissues that have been ashed at 500 °C, whereas quantification of soluble Pi is performed with tissues extracted under mild acid conditions (which preserves acid-labile phosphate ester bonds).
[摘要]  对于无机磷酸盐(Pi)的简单,快速和灵敏的比色微量测定依赖于在酸中通过钼酸铵-Pi络合物还原后形成的钼蓝络合物在660nm处的吸收。 测定总Pi的方法使用在500℃下灰化的植物组织,而可溶性P 1的定量是在温和酸条件下(其保留酸不稳定的磷酸酯键)提取的组织进行。

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