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Sigma® cell culture plate

细胞培养板

公司名称: Sigma-Aldrich
产品编号: SIAL0596-50EA
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NMDA-induced Excitotoxicity and Lactate Dehydrogenase Assay in Primary Cultured Neurons
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2013-11-05
[Abstract]  N-Methyl-D-aspartic acid receptor (NMDAR)-mediated excitotoxicity is thought to contribute to the pathogenesis of a large number of chronic neurodegenerative disorders (such as Alzheimer’s and Huntington’s diseases to mental illnesses) in addition to acute brain insults such as stroke and brain trauma. Understanding the mechanisms underlying NMDAR-mediated excitotoxicity may lead to development of novel therapeutics for treating neurological diseases. Stimulation of primary cultured neurons with excessive NMDA is widely used as an in vitro model for studying NMDAR-mediated ... [摘要]  认为N-甲基-D-天门冬氨酸受体(NMDAR)介导的兴奋性毒性被认为有助于大量慢性神经退行性疾病(如阿尔茨海默病和亨廷顿疾病对精神疾病)的发病机制,除了急性脑损伤如中风和脑外伤。了解NMDAR介导的兴奋性毒性的机制可能导致治疗神经系统疾病的新疗法的发展。具有过量NMDA的原代培养神经元的刺激被广泛用作研究NMDAR介导的兴奋性毒性的体外模型,其允许仔细解剖兴奋性毒性神经元死亡的详细细胞机制。
乳酸脱氢酶(LDH)是可将NAD转化为NADH的细胞质酶。当质膜完整性受损时,LDH从细胞释放到培养基中。因此,释放的LDH的量代表细胞死亡的程度。在我们目前的研究中,使用从Sigma-Aldrich获得的体外毒理学测定试剂盒测量细胞外LDH水平。该LDH测定的基础是:1)LDH将NAD还原成NADH,2)然后将所得NADH用于四唑鎓染料的化学计量转化,和3)所得着色化合物通过分光光度计酶标仪在波长为490nm。细胞死亡率以处理组的吸光度与对照组的吸光度之间的百分比(%)表示。

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