| Primary Explosive Blast-induced Traumatic Brain Injury Model in PC12 Cell Culture
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Author:
Date:
2016-08-20
[Abstract] While it is understood that structural damage occurs at the cellular level from the traumatic brain injury event, the effect on functional activity remains largely unknown. Simplified models such as in vitro models of primary explosive blast are critically needed to deconvolute mechanisms of cellular damage. This protocol details an in vitro indoor experimental system setup (Zander et al., 2015) using real military explosive charges to more accurately represent battlefield blast exposure, and probe the effects of primary explosive blast on dissociated neurons.
[摘要] 细胞毒性CD8 + T细胞能够通过其T细胞受体（TCR）与主要组织相容性复合物（MHC）分子呈递的小免疫原性肽（抗原）之间的特异性相互作用特异性识别和杀死靶细胞。抗原特异性细胞毒性T细胞的抗原识别能力和体外裂解活性可以在所谓的铬51（<51> Cr）释放测定中功能性评估，其是几乎50年前在我们的机构中发展起来的（Brunner等人，1968年）。放射性标记的内源性抗原呈递缺陷的细胞[例如，用于抗原呈递（TAP）缺陷型T2细胞的转运蛋白]，并用感兴趣的MHC稳定转染（例如 ，HLA-A2 sup + +）通常在这个4小时测定期间用作靶标。或者，内源性呈递免疫原性抗原的Cr标记的病毒感染或肿瘤细胞系可以作为靶细胞（例如，用于评估肿瘤识别）。 在肽滴定测定（部分A）中，用抗原性肽的系列稀释物对放射性标记的靶细胞进行脉冲，并在效应物（例如，CD8 + T细胞克隆）与靶细胞（ Cr-T2细胞）比（E:T）为10:1的混合物在96孔V型底板中37℃温育4小时。在肿瘤杀伤试验（B部分）中，将细胞毒性CD8 ...
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| Harvest and Culture of Mouse Peritoneal Macrophages
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Author:
Date:
2013-11-20
[Abstract] Peritoneal macrophages are used as primary macrophages in lots of studies, mainly because they are easy to obtain. Injection of thioglycollate broth i.p. induces inflammatory responses and elicits large numbers of macrophages. This protocol can be used for harvesting resident or thioglycollate-elicited peritoneal cells. Peritoneal macrophages are non-adherent in situ and when they are cultured in dishes, they become adherent so that macrophages may be separated from other types of cells in peritoneal cavity.
[摘要] 腹膜巨噬细胞在许多研究中用作初级巨噬细胞，主要是因为它们容易获得。 注射巯基乙酸盐肉汤i.p. 诱导炎症反应并引发大量的巨噬细胞。 该方案可用于收获驻留的或巯基乙酸盐诱发的腹膜细胞。 腹膜巨噬细胞是原位非粘附的，并且当它们在培养皿中培养时，它们变得粘附，使得巨噬细胞可以与腹膜腔中的其它类型的细胞分离。
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