Beckman Optima TL 100 Ultracentrifuge

Beckman Optima TL 100超速离心机

公司名称: Beckman Coulter

产品编号: Optima^TM TL 100

Preparation and Purification of β-1,3-glucan-Linked Candida glabrata Cell Wall Proteases by Ion-Exchange Chromatography, Gel Filtration, and MDPF-Gelatin-Zymography Assay

Pirjo Pärnänen, Timo Sorsa, Taina Tervahartiala and Pirjo Nikula-Ijäs

Published online: 2024-03-20

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Fractionation by Ultracentrifugation of Gram Negative Cytoplasmic and Membrane Proteins

Author:

Sara M Sandrini, Richard Haigh and Primrose P. E. Freestone,

Date:

2014-11-05

[Abstract] Protein fractionation is a useful separation process which divides membrane proteins (including those located in the outer and inner membrane) and cytoplasmic proteins into discrete fractions. Fractionation of proteins can simplify analysis of the numbers of proteins present, and therefore make easier to characterize any environmentally or mutation induced changes in expression profiles, or changes in protein strucutre resulting from post-translational modification. This protocol is derived from Haigh et al. (2013) and it is specific to Gram negative bacteria. [摘要] 蛋白分级是一种有用的分离过程，其将膜蛋白（包括位于外膜和内膜中的那些）和胞质蛋白分成离散的级分。蛋白质的分馏可简化对存在的蛋白质数量的分析，因此使得更容易表征任何环境或突变诱导的表达谱的变化或由翻译后修饰导致的蛋白质结构的变化。该方案衍生自Haigh等人（2013），并且其特异性针对革兰氏阴性细菌。

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Beckman Optima TL 100 Ultracentrifuge

Beckman Optima TL 100超速离心机

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