| Electroshock Induced Seizures in Adult C. elegans
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Author:
Date:
2017-05-05
[Abstract] The nematode Caenorhabditis elegans is a useful model organism for dissecting molecular mechanisms of neurological diseases. While hermaphrodite C. elegans contains only 302 neurons, the conserved homologous neurotransmitters, simpler neuronal circuitry, and fully mapped connectome make it an appealing model system for neurological research. Here we developed an assay to induce an electroconvulsive seizure in C. elegans which can be used as a behavioral method of analyzing potential anti-epileptic therapeutics and novel genes involved in seizure susceptibility. In ...
[摘要] 线虫秀丽隐杆线虫是解剖神经系统疾病分子机制的有用的模型生物。而雌雄同体线虫仅包含302个神经元，保守的同源神经递质，更简单的神经元电路和完全映射的连接体使其成为神经学研究的吸引人的模型系统。在这里，我们开发了一种诱发电刺激性癫痫发作的检测方法。线虫可用作分析潜在的抗癫痫药物和涉及癫痫发作敏感性的新基因的行为方法。在该测定中，当电流通过液体时，将蠕虫悬浮在水溶液中。在休克开始时，蠕虫会短暂瘫痪和抽搐，并在恢复正常的正弦运动后不久。运动恢复的时间被用作从癫痫发作恢复的指标，可以通过掺入改变神经元和肌肉兴奋性的药物来减少或延长。
背景 我们有兴趣使用强大的遗传模型，即秀丽隐杆线虫，开发可以容易地被药理学操作的电惊厥发作测定。无脊椎动物模型已被用于癫痫发作研究几十年（Lee和Wu，2002），但是没有专门研究电刺激性癫痫发作的方案。线虫。过去，多组已经开发出响应于化学前同质醇如GABA A受体阻断剂戊戊四唑（PTZ）和微毒素（PTX）以及乙酰胆碱酯酶抑制剂涕灭威分析麻痹的方法（Williams等人，2004; Vashlishan等人，2008）。虽然这些方法通常分析麻痹的时间，我们的方法量化了从电击诱发的癫痫发作恢复所需的时间（Risley等，2016）。
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| Mouse Corneal Stroma Fibroblast Primary Cell Culture
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Author:
Date:
2016-10-05
[Abstract] This protocol is developed for primary cell culture of cornea stromal keratocytes isolated from neonatal mouse eyeballs. It provides an optimal condition to isolate stromal keratocytes which maintain high viability for cell culture.
[摘要] 该协议是为从新生小鼠眼球分离的角膜基质角膜细胞的原代细胞培养而开发的。 它提供了一个最佳条件，以隔离基质角膜细胞，保持细胞培养的高生存力。
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| Invadopodia Detection and Gelatin Degradation Assay
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Author:
Date:
2013-12-20
[Abstract] This protocol is designed to quantify invadopodia formation and activity. Invadopodia are protrusive structures elaborated by cancer cells that mediate cell attachment and remodeling of the extracellular matrix. These structures contribute to the ability of cancer cells to invade and metastasize. In this protocol, both the presence of invadopodia and their activity is simultaneously assessed and quantified by a fluorescent microscopy-based assay.
[摘要] 该协议旨在量化侵袭过程的形成和活性。 Invadopodia是介导细胞附着和细胞外基质重塑的癌细胞阐述的突出结构。 这些结构有助于癌细胞入侵和转移的能力。 在该方案中，通过基于荧光显微镜的测定法同时评估和定量侵袭多巴的存在及其活性。
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