| Phenol-based Total Protein Extraction from Lily Plant Tissues
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Author:
Date:
2015-02-20
[Abstract] The phenol-based total protein extraction method is unique in that water-soluble components such as polyphenolic compounds and nucleic acids can be easily removed. Thus, total protein is free from contaminants and allows for high quality two–dimensional gel electrophoresis. The phenol-based extraction of total protein was used in various lily organs and may likely apply to other plants whose content of polyphenolics is high (Note 1). An additional advantage of this extraction method is that nucleic acids can be easily removed and thus, avoid adverse effects of nucleic acids on protein ...
[摘要] 酚类总蛋白提取方法的独特之处在于可以容易地除去多酚化合物和核酸等水溶性成分。 因此，总蛋白不含污染物，并允许高质量的二维凝胶电泳。 总蛋白的苯酚提取用于各种百合器官，可能适用于多酚含量高的其他植物（注1）。 该提取方法的另一个优点是，可以容易地除去核酸，从而避免核酸对凝胶中蛋白质分辨的不利影响。 这种方法从Hurkman和Tanaka（1986）的修改。
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| Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
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Author:
Date:
2014-04-20
[Abstract] This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes.
[摘要] 该方案是为研究减数分裂染色体扩散而开发的以前方案的更详细的版本（Yang等人，2011;Bolaños-Villegas等人，2013）。 减数分裂染色体扩散可用于确定染色体配对和分离中缺陷的存在。 该方案还描述了如何使用用于标记染色体的着丝粒探针进行荧光原位杂交实验。
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