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Author:
Date:
2014-04-20
[Abstract] Exosomes are small membrane vesicles of endocytic origin secreted into the extracellular environment from a variety of different cells, and are thought to play important roles in intercellular communications. Here, we provide a useful protocol to purify the exosomes released from cell lines using sucrose gradient centrifugation. In this protocol, we also applied a red-fluorescent lipophilic dye, DiI, which is incorporated in the outer membrane of exosomes. This fluorescently labeled exosomes allow us to visualize individual exosomes by a confocal laser scanning microscope.
[摘要] 外来体是从各种不同细胞分泌到细胞外环境中的内吞起源的小膜囊泡，并且被认为在细胞间通讯中起重要作用。 在这里，我们提供了一个有用的协议，以纯化使用蔗糖梯度离心从细胞系释放的外来体。 在这个协议，我们还应用红色荧光亲脂染料DiI，其被并入外来体的外膜。 这种荧光标记的外来体允许我们通过共聚焦激光扫描显微镜可视化单个外来体
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