| Quantification of the Composition Dynamics of a Maize Root-associated Simplified Bacterial Community and Evaluation of Its Biological Control Effect
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Author:
Date:
2018-06-20
[Abstract] Besides analyzing the composition and dynamics of microbial communities, plant microbiome research aims to understanding the mechanism of plant microbiota assembly and their biological functions. Here, we describe procedures to investigate the role of bacterial interspecies interactions in root microbiome assembly and the beneficial effects of the root microbiota on hosts by using a maize root-associated simplified seven-species (Stenotrophomonas maltophilia, Ochrobactrum pituitosum, Curtobacterium pusillum, Enterobacter cloacae, Chryseobacterium ...
[摘要] 除了分析微生物群落的组成和动态外，植物微生物群落研究的目的在于了解植物微生物群落组装的机制及其生物学功能。在这里，我们描述了通过使用与玉米根相关的简化的七种物种（嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia），）来研究细菌种间相互作用在根微生物群组装中的作用以及根菌群对宿主的有益影响的程序。 Ochrobactrum pituitosum ，Curtobacterium pusillum ，阴沟肠杆菌， Chryseobacterium indologenes ，<在我们以前的工作中描述过的假单胞菌（pseudomonas putida））合成细菌群落。表面灭菌的玉米种子在含有多种细菌的悬浮液中浸泡后，在基于双管生长室的非生物体系中生长。通过对7种菌株中的每种菌株选择性培养基的培养依赖性方法追踪定殖在玉米根部的细菌群组成的动态。通过监测社区结构的变化来评估细菌相互作用对社区组装的影响。通过量化（1）真菌植物病原体，种子表面上的fusarium="" verticillioides和（2）幼苗枯萎的严重程度来评估简化的7种群落的植物保护效果真菌引起的疾病，在细菌群落的存在和不存在的情况下。我们的方案将作为研究实验室条件下植物="" -=""> ...
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| Minimal Bactericidal Concentration for Biofilms (MBC-B)
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Author:
Date:
2014-05-05
[Abstract] A biofilm is a multicellular consortium of surface associated microbes surrounded by a hydrated, extracellular polymer matrix. The biofilm matrix plays a critical role in preventing desiccation, acquiring nutrients, and provides community protection from environmental assaults. Importantly, biofilms are significantly more resistant to antimicrobials relative to their free-swimming counterparts. The level of antimicrobial tolerance is influenced by a number of factors, including genetic/adaptive resistance mechanisms, stage of biofilm development, and pharmacokinetics of the antibiotic. Here, ...
[摘要] 生物膜是由水合的细胞外聚合物基质包围的表面相关微生物的多细胞聚生体。生物膜基质在防止干燥，获得营养物质和提供社区保护免受环境攻击中起关键作用。重要的是，相对于其自由游泳的对应物，生物膜显着地更抗抗微生物剂。抗微生物耐受性的水平受许多因素影响，包括遗传/适应性抗性机制，生物膜发育阶段和抗生素的药代动力学。在这里，我们描述了一种基于体外微量滴定的测定法，用于定量短曝光时间（2小时）的生物膜（MBC-B）的最小杀菌浓度。该暴露时间显着短于传统方案中描述的标准过夜和24小时治疗。开发该测定法以接近抗生素在其被代谢，被宿主蛋白螯合或消化之前在一次性治疗期间可用的时间。
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| Microfluidic-based Time-kill Kinetic Assay
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Author:
Date:
2014-05-05
[Abstract] In many environments, bacteria favor a sessile, surface-attached community lifestyle. These communities, termed biofilms, are ubiquitous among many species of bacteria. In some cases, biofilms form under flow conditions. Flow chambers, and in particular microfluidic channels, can be used to observe biofilm development and physiological effects while varying nutrient conditions, flow velocities, or introducing antimicrobials to the biofilm in real time. Here, we describe a microfluidic-based kill-kinetics assay for the observation of antimicrobial effects on biofilms under flowing conditions. ...
[摘要] 在许多环境中，细菌喜欢固着的，表面附着的社区生活方式。 这些社区，称为生物膜，在许多物种的细菌中是普遍存在的。 在一些情况下，在流动条件下形成生物膜。 流动室，特别是微流体通道，可以用于观察生物膜的发展和生理效应，同时改变营养物条件，流速，或实时地将抗微生物剂引入生物膜。 在这里，我们描述了一种基于微流体的杀菌动力学测定法，观察在流动条件下对生物膜的抗微生物效应。
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